NF-L protein implicate the tail domains of NF-M and NF-H as major determinants of the high proteolytic resistance and exceptionally long half-lives of NF and, thus, as key regulators of NF number in axons. Our results demonstrate a critical function for the C-terminal domains of NF-M and NF-H subunits in stabilizing the axonal NF network by increasing its resistance to proteolysis, thereby expanding the content of neurofilaments in axons. The carboxyl terminal tail domains of NF-H and NF-M have LDN-193189 previously been shown to form fine long cross-bridges that interconnect adjacent NFs and NFs and microtubules. By means of a varied group of cytoskeleton-associated proteins the NF network is interlinked to the microtubule, actin and spectrin cytoskeletal elements to establish a three-dimensional lattice in the axon. This stationary support structure, maintained by transported cytoskeletal elements or their precursors, serves as a platform for transport and provides the tensile support for axon caliber expansion required for electrical conductance along axons. When the C-terminal domain of both NF-H and NF-M are eliminated in NF-H/M tail deletion mice, the NF cytoskeleton loses most of its cross-bridging and appears highly disorganized. We observed that loss of the Cterminal regions had negligible effects on entry and transport of NFs in axons but increased the rate of disappearance of stationary NFs from axons leading to a markedly decreased NF content along optic axons. Although the factors that contribute to the turnover of the NFs are not clear, the unstable NF networks in NF-H/M tail deletion mice may be more prone to proteolysis in the axon by cellular proteases such as calpains. Calpains have been shown to be involved in turnover of NFs, particularly when they are relatively poorly phosphorylated at their C-terminal ends. Our study resolves the longstanding issue of whether C-terminal domains of NF-M and NF-H regulate the axonal transport of NF by demonstrating that NFs that lack these domains exhibit the same transport velocities along axons as NFs with intact. Nevertheless, the paradigm persists that growth is more sensitive to a plant’s resource environment than is photosynthesis, and decreased growth with concomitant increases in defenses has been documented many times. Additionally, the cost of each HRV was cheaper than other cardiovascular autonomic function tests in China. The risk score method is used for a primary medical care setting and for a layperson to perform self-assessment to identify high-risk people. The high-risk individuals were generally recommended to perform a HRV test that is available in most primary health care settings in China now. In addition, the high-risk individuals will benefit from receiving health education and changing to healthy lifestyles at an early stage so as to prevent or delay the onset of CAN. The present study provides a risk score based on a specific population in China. The predictive performance and discriminative ability of the score is not confirmed in other ethnics. Nevertheless, future researchers might investigate more about the generalizability of these score rules across countries.