RNA gene expression ratios dropped relative to the two and six month checkpoints, though the differences between these ratios are not statistically significant. qPCR carries with it an inherent variability, which may explain the differences seen. Small pipetting errors can result in highly varied Ct values after exponential DNA amplification. Running samples in triplicate and averaging the results helps to reduce possible errors but qPCR should not be relied on completely. Individual samples varied in calculated TBP Ct value across the time points in ways that would not be predicted from mere difference in storage length. Ct numbers would not be expected to go down after an RNA sample is stored for more time, but for some of the samples tested, Ct numbers dropped from month two to month six or from month six to month 12. The fact that the measured desiccated-to-frozen gene expression level ratios as measured by qPCR were lower at the 12 month time point could well be accounted for by the qPCR Ct variability. Regardless of any possible errors or variability, Ct values for TBP expression remained in the low to mid 30 s throughout the yearlong study for both desiccated and frozen samples representing preservation of mRNA. After being stored for 76 days at either room temperature in a desiccated state or at 280uC, RNA aliquots of two whole RNA extracts stored at these conditions were compared by RNA sequencing on gene expression of 14,114 genes. Transcriptomewide expression profiles from RNA samples which were stored desiccated or frozen were very similar, indicating that the RNA stored by the desiccated method maintained the same gene expression profiles as the RNA stored in the traditional manner. In summary, desiccation appears to be a very promising technique for long term RNA storage. Storage in a desiccated state preserves RNA integrity and allows for robust downstream standard and next-generation transcriptomic analysis, and as such should be seriously considered in any modern biotechnical institute that deals with RNA. As desiccated room temperature storage has been shown to be effective at preserving high quality RNA, desiccation of RNA may also one day supplant ULT freezers for RNA storage, and would thus reduce laboratory reliance upon carbon-based power grids. Therefore, use of RNA desiccation techniques may save research groups energy costs, while simultaneously reducing their carbon footprint and significantly allaying the present fears associated with the real possibility of freezer failures. The rapid proliferation of cancer cells is associated with a metabolic shift from mitochondrial oxidative phosphorylation to aerobic glycolysis, a phenomenon known as the Warburg effect. This process is dependent on the enzyme lactate dehydrogenase A, which produces NAD + and lactate. The NAD + produced in this manner may function as a cofactor for the SIRT1 enzyme. If SIRT1, as a metabolic sensor, is a link between metabolism and carcinogenesis, then known modulators of SIRT1 activity could be exploited for preventative or therapeutic benefit. Identified modulators of SIRT1 activity include Ganoderic-acid-G caloric restriction, a high fat diet, and a number of chemical compounds Clofentezine including the plant phytoalexin, resveratrol. In mammals, caloric restriction can delay the onset of various ageing-related diseases including cancer. CR has been associated with lifespan extension and in mice this phenomenon is thought to be dependent on SIRT1 activity. This suggests that Sirt1 may have the properties of a tumor suppressor gene and several studies have suggested that this may be the case. Paradoxically, many studies suggest that Sirt1 is an oncogene. Still other studies found that Sirt1 has no effect on oncogenesis.