The hyperaemia zone carries less risk of necrosis and usually recovers well. Overall, wound progression may result in hypertrophic scarring, wound contractures, infections, sepsis and mortality without appropriate intervention. Therefore, preventing wound progression is a challenge for clinical therapy. The mechanisms of burn wound progression include vasoconstriction/vasodilation, injury by oxygen free radicals, hypoper fusion and micro thrombosis, all of which result in inflammatory cascade activation and even cell death.Autophagy, previously regarded as type II programmed cell death, has also been reported to be involved in early wound progression after a burn, although it is unclear whether this response is beneficial or detrimental. The small gas molecule hydrogen is able to penetrate the cell membrane and enter the cytosol, mitochondria, and nucleus and selectively scavenge hydroxyl radicals and Sulfadoxine peroxynitrite anions of reactive oxygen species, there by preventing their interference in normal metabolism and signal transmission.Moreover,H2 has protective effects on several types of oxidative stress-induced organ damage, such as early brain injury after subarachnoid haemorrhage, radiation-induced injury, sepsis, and ischaemia-Salbutamol Sulfate reperfusion injury in multiple organs, by suppressing oxidative stress and apoptosis, inhibiting inflammatory cell infiltration and regulating pro-inflammatory cytokine expression and inflammation/apoptosis-related signaling pathways. With respect to skin wounds, previous in vitro and in vivo studies haveindicatedthatH2 could protect skin flap or diabetic wounds from is chaemia/reperfusion or hyper glycaemia-induced injuries, respectively. Hence, we hypothesised on the possible impacts of H2 on burn wound progression and all experiments were designed to evaluate potential mechanisms of action and regulation. Following our prior experiences and previous work, we selected hydrogen-rich saline as a carrier of molecular hydrogen via intraperitoneal administration as an effective and convenient therapy in this study.