A major limitation for these studies is the fact that for most of ovarian cell lines their origin is poorly defined and owing to inadequate characterization it is not known which distinct histological or molecular subtype is represented. We here describe an extensive and uniform characterization of a collection of 39 ovarian cancer cell lines commonly used for in vitro studies. We identified histological as well as morphological subtypes which associate with clinical pathological characteristics of ovarian carcinomas as well as prognosis. Furthermore, the morphological subtypes associate with Ginsenoside-F3 the molecular subtypes identified by Tothill et al.. In summary, these results can serve as a guide to select appropriate cell lines representing different histological and molecular subtype of ovarian cancer for in vitro therapeutic studies. All cell lines were initially cultured using the medium and supplements as recommended by the suppliers. In contrast to other studies, we cultured all cell lines under the same culture conditions to avoid biases due to Ginsenoside-F2 varying concentrations of supplements within different media or different percentages of serum. Luminal cytokeratin and EpCAM are strongly correlated and absent expression of both markers was most commonly seen in Round cell lines that were in general very sensitive to most therapeutics. If the four Round cell lines are excluded, the only significant association observed was between Oxaliplatin response and expression of luminal cytokeratin’s, indicating that this small group of cell lines is mainly causing other associations. We next used the Spearman rank correlation to test for correlation between the GI50 values and the expression of each mRNA or microRNA in order to identify genes and microRNAs associated with response to therapy. Using these criteria, we deduced the putative histological subtype for the 39 cell lines as 20 non-serous and 19 serous cell lines which included 14 high-grade serous and one low-grade serous line. We used the expression data for 267 malignant ovarian carcinomas to determine the clinical significance of the morphological subtypes seen in our cell lines. After averaging of duplicate probes, the hierarchical clustering based on the expression of the 1141 morphological specific genes showed two tumour clusters, resembling 1) the Spindle-shaped cell lines and 2) the Epithelial and Round cell lines. Patient and clinicopathological features are given in Table 2 as well as their association with the two morphological clusters.