Acetone is a widely accepted solvent for preparation of PLGA nanoparticles due to its miscibility with water in all proportions. Spontaneous diffusion of acetone in water creates an interfacial turbulence resulting in formation of particles in nanoscale. This principle was experimented for entrapment of Sb in PLGA. Pluronic F-127 was used as stabilizer. Pluronics are a group of Cussosaponin-C ABA triblock copolymers having poly ethylene glycol as outer block and hydrophobic poly as middle block and can help in modifying the fate of synthesized nanoparticles. Pluronic F-127 has also been approved recently by the US FDA for application in drug delivery devices. The new nano-device designed with Sb as a payload was studied in streptozotocin induced diabetic model as one remedial alternative in systemic hyperglycemia. Chitosan quintessence on CSbnp was Hederacolchiside-A1 measured using a chitosan electrostatic interaction reaction with alizarin red dye. A standard graph from chitosan and alizarin reaction extinction recorded at 571 nm was used to estimate chitosan mass content both in the primary stock solution and in CSbnp preparation supernatant after centrifugation. The difference in mass was considered for estimation of chitosan coating. Experiments were run in triplicate in each case and the data recorded in percentage entrapment from three separate batch preparations. Nanoparticulation of biopharmaceutic class IV type compounds is one strategy that is helpful in dissolution improvements, specific administration and facilitated bioactivity. PLGA nanocarriers with Sb payload were prepared by solvent diffusion of acetone in water environment. Diffusion of solvent in water phase resulted in rapid particle formation. As the solvent rapidly diffuses into the water phase, an interfacial turbulence results which, can cause deposition of polymer at the transient acetone/water interface. Some advantages of this technique are mild preparative conditions, avoidance of high stress force and an apparent ease in future scale ups. US FDA approved tri block polymer pluronic F-127 was used as a stabilizer in place of common agent PVA.