The good match between the results in training and cross-validation datasets provides further support to the model. Whereas previous studies AG-09/1 reported correlations between metabolic profile and different CVD risk factors and disease states such as insulin resistance, diabetes, obesity, the present study represents the first description of metabolic profiles of microalbuminuria in a general population. The differential metabolic profiles show that branched amino acids are reduced in microalbuminuria. The statistical significance of different spectral regions containing resonances of BCAA and related metabolites, like 3-OH-isovalerate, supports the association. BCAA can act as signaling molecules in many processes. Although many studies report increased BCAA levels in diabetes and insulin resistance, the association with microalbuminuria has not been previously explored. Early studies showed that idiopathic portal hypertension correlates to decreased levels of leucine, isoleucine and valine. Diet-induced insulin resistant obese mice also display a depletion of BCAA serum levels. The interpretation of these findings is complex because fasting status, diet, exercise and basal metabolism affect BCAA levels in diverse ways. The combined effect of lipids and BCAA seems pivotal in a complex network of interactions involving muscle, adipose, liver and brain metabolisms. The microalbuminuric pattern, mainly in hypertension and/or diabetes, was also associated to alterations in glucose metabolism, lipid b-oxidation and the BIBP 3226 tricarboxilic acid cycle. These are central metabolic cores for all eukaryotic cells. We report changes in lipids, glucose, pyruvate, lactate, alanine and glutamine which suggest important shifts in energy metabolism. However, the interpretation of these changes in relation to develop microalbuminuria is unclear. Different studies reported changes in different directions for these metabolites in obesity and related complications. In the present study, glutamine, the most abundant amino acid in plasma, is also associated to microalbuminuria. Glutamine can be produced in the TCA cycle via 2- oxoglutarate and glutamate. It is also an important precursor of urea. As a consequence, glutamine plays a pivotal metabolic role, which can be affected by alterations in both TCA cycle and urea metabolism. Finally, choline, containing compound resonances associated to microalbuminuria, was also observed. Although choline is involved in multiple metabolic pathways, it has a predominant role in cell membrane integrity, methyl metabolism and lipid-cholesterol transport.