Expression was stronger at the medial portions of the apex of the stage 7 and 8 gynoecial primordia relative to more basal positions. Expression continues in the apical medial NVP-BEZ235 domain into stage 10. Expression is also detected in ovule primordia as they arise at stage 8 and continues GSK212 throughout ovule development. Expression in ovule primordia at stage 11 was strongest in the chalazal portions of the ovule. Expression in the ant single mutant tissue at this stage appeared reduced, suggesting that ANT may regulate the expression of VDD in the chalazal portions of the ovule. Strong expression was also detected in tapetal cells of the anther at stage 9. Expression of At3G17010 in stamen and carpel primordia has been previously reported. We first detected expression of AT3G17010 starting at late stage 4 or early stage 5 in the stamen primordia as they arise. Within the gynoecium expression can be detected preferentially in the medial domain as early as stage 6. AT3G17010 continues to be preferentially expressed subepidermally within the medial domain through stage 8 and is strongly detected in the medial domain at apical positions of the stage 8 gynoecium. Expression is detected in the ovule primordia during stage 9 and 10 in subepidermal layers. The AT3G19184 transcript is detected throughout the inflorescence meristem and throughout stage 1�C4 floral meristems. During stage 5 expression of AT3G19184 is strongest in stamen and petal primordia as they arise. Expression is detected throughout stage 6 and 7 gynoecia. It is strongly detected at the apical regions of stage 7 gynoecia, particularly in medial positions. Expression in stage 8 gynoecia is strongest in ovule primordia as they arise. Expression in stage 7 stamen primordia is detected strongly in the precursors of the archesporial and tapetal cells and is later expressed in microspores and tapetal cells during stage 9. Expression of AT5G57720 in stamen and carpel primordia was previously reported. We first detect expression of AT5G57720 during early stage 4 as a ring of expression that appears to mark whorl three positions just interior or adaxial to the sepal primordia. During stage 6 AT5G57720 is detected in the gynoecium in the medial domain, most strongly in abaxial positions. During stage 7 AT5G57720 is detected throughout the medial domain of the gynoecium and continues to be detected in adaxial portions of the medial domain during stage 8. AT5G57720 is detected in ovule primordia as they arise during stage 8 and continues to be expressed in the megaspore mother cell and in nucellar portions of the ovule through stage 12. AT5G57720 is also detected strongly in stamen tapetal cells during stage 9. PERIANTHIA expression patterns have been previously published. PAN is detected strongly in the stage 6 gynoecial primordium within the medial domain.