For example on these two varieties BPH spent around 90% of time not penetrating or in pathway. However no N4-b behaviour was observed. In contrast, BPH feeding on Azucaena showed the highest AMN107 citations duration of phloem ingestion over this period. Table 5 shows the average frequency of all EPG waveforms in each h over the last 5 h of experiment. ANOVA revealed that phloem ingestion and derailed stylet mechanics were highest in TN1, IR694 and Nipponbare. Cluster analysis using Ward��s method based on Euclidean Distance was performed using 56 activities derived from EPG Cabozantinib waveform duration and frequency for the last 5 h of the 12 h feeding period. Fundamentally, this multivariate method involves making pairwise comparisons of all objects , and then classifying them according to an average linkage method and illustrating the object relationships in a dendrogram . Therefore, the real objective of this analysis is to summarize overall data for classification of resistant and susceptible varieties. Total and average honeydew data for the same last 5 h of feeding were also included in the analysis . The resulting dendrogram divided the 12 rice varieties into three main groups at a 0.15 semi partial R square value. Group 1 included Azucaena, TN1, Nipponbare and IR694. This group showed the greatest distance from the other two groups namely group 2 – Fujisaka, IR758, MR219 and MR232, while Rathu Heenathi, IR64, Babawee and F1 formed a third group. In Univariate analysis , 38 out of 56 activities showed highly significant differences between varieties. These characters mostly related to non penetration, pathway, N4-b and honeydew drop, and the resistance versus susceptibility can clearly be distinguished for all 12 varieties. Further analysis of the common characteristics of the three groups identified by cluster analysis demonstrated that resistance was associated with high percentage duration of NP, pathway, N5, N6 and N7 EPG waveform characters . In contrast the susceptible group was associated with the longest duration of N4-b . Interestingly N4-a pattern waveform did not statistically differentiate between those groups. In this study we have been able to characterize BPH feeding behaviour using DC- based electrical penetration graph , and use this to screen 12 rice varieties of differing resistance, facilitating the efficient and detailed classification of rice germplasm for insect resistance.