Consequently our knowledge advise that for scientific strains serotype-independent elements are frequently just as powerful influences on C3b/iC3b deposition as capsular serotype. As effectively as distinctions in C3b/iC3b deposition between strains with distinct STs, there have been also differences for strains with the same ST and capsular serotype. As a result getting an precise median degree of C3b/iC3b deposition for every single serotype utilizing information attained from a fairly modest amount of consultant strains is not really achievable, as employing serotype and ST by yourself may not adequately determine a selection of consultant strains. Instead comparing phenotypes this kind of as enhance sensitivity amongst every capsular serotype will require investigating a extremely massive amount of clinical strains for every serotype or employing capsular switched strains. A number of mechanisms might describe variation in C3b/iC3b deposition impartial of capsular serotype. The most evident would be differences in capsule thickness or antibody activity between strains, and we have investigated these possibilities for chosen strains demonstrating massive variations in C3b/iC3b deposition. We identified no distinctions in capsule thickness between serotype 6B and 23F strains with large variations in sensitivity to ALK5 Inhibitor II citations complement, and antibody to capsular polysaccharide ought to have equivalent outcomes for strains with the exact same capsular serotype. Overall IgG binding, which includes binding to protein and other non-capsular antigens that might range in expression between strains of the exact same capsular serotype, did weakly correlate with C3b/iC3b deposition. Nevertheless, massive differences in C3b/iC3b deposition among some strains with the identical serotype persisted when IgG was depleted by cleavage with IdeS, indicating that there must be extra mechanisms impacting C3b/iC3b deposition. Serotypes with large versions between strains in the C3b/iC3b deposition final results tended to be people that have formerly been explained as more genetically diverse, while those with significantly less variation in C3b/iC3b results had been a lot more clonally associated.