Therefore, it is more important to determine the ignored knowledge than the information growth itself. Swanson developed and implemented a novel tool to mine the existing knowledge base for unreported or underreported relationships, and resurrect previously published but neglected hypotheses, a process known as literature-based discovery. This process functions as a way of connecting 2 seemingly unrelated findings, otherwise stated in this form, then the hypothesis is strongly suggested. Although this approach does not provide a conclusive proof, the discovery is, in itself, very helpful in uncovering previously unknown relationships. Further, it can help the investigators access context and mine knowledge that might not be revealed using a traditional search. In the present study, we performed a 2-step approach to simulate Swanson��s literature-based discovery methodology in 2 fields of biological research literature that are normally not bibliographically connected: gastric cancer and psychology. Gastric cancer is one of the most commonly diagnosed cancers, the second leading cause of cancer-related death worldwide, and a serious public health problem. Previous research has also demonstrated that a variety of psychological factors can have a considerable effect on physical diseases. However, the relationship between gastric cancer and psychology has previously been neglected. Therefore, these 2 fields of research were selected with the goal of finding a neglected common connection. The 2-step discovery process generated a hypothesis about the correlation between anandamide and gastric cancer and provided a BMS 493 possible common Binucleine 2 molecular network which may mediate the effects. The hypothesis was then investigated experimentally. Anandamide was found to inhibit growth in 4 gastric cancer cell lines, including BGC823, SGC7901, AGS, and N87. Flow cytometry data demonstrated that the presence of anandamide induced G2/M cell cycle arrest in AGS and N87 cells. Furthermore, we confirmed that anandamide can act to regulate cell cycle associated genes, including CHEK1, CDKN1A, CDKN2A, obtained from the closed discovery process. Collectively, these data indicate that anandamide affects the cell cycle distribution of gastric cancer cells by regulating the B-terms cell cycle regulators, a hypothesis that was validated experimentally in this study, thereby providing investigators with an alternate view regarding the role of anandamide.