Since the relative role or importance of TORC1 in yeast filamentous growth is still fairly unclear, in this study we specifically focused only on this subset of KRX-0401 157716-52-4 identified proteins. Using prototrophic yeast strains from two different genetic backgrounds, we present data suggesting that nitrogen starvation-dependent diploid pseudohyphal growth may be regulated by the TORC1. This may represent one means through which budding yeast distinguishes between the numerous diverse inputs that induce filamentous differentiation upstream of the PKA, Snf1 and MAPK pathways. Our work also suggests the CEN.PK strain background will be useful for the exclusive study of nitrogen starvation-dependent diploid pseudohyphal growth. We developed a reliable, efficient method to biochemically isolate TORC1 from yeast grown under different nutrient conditions. Our immunopurification yielded TORC1 containing Kog1p and Tor1p at stoichiometrically similar levels, as well as a number of specifically associated proteins that do not co-purify in control samples. A number of these associated proteins were excised from SDS-PAGE gels and identified using standard nano-HPLC/MS/MS methods, resulting in the identification of several new as well as known TORC1 components. When classified by known function, there appeared to be four broad functional groups of proteins that interact with the yeast TORC1 based on our purification. These included the translational machinery and ribosome biogenesis, RNA binding proteins potentially related to translation, endocytosis/ eisosome, as well as several proteins involved in filamentous growth. TORC1 is known to be a major regulator of the translational machinery, RNA regulation and ribosome biogenesis. Many of the proteins we identified in the first three groups had not been studied previously as TORC1 components or TORC1-regulated proteins. We tested the effect of individual deletion mutants of these proteins on yeast growth in the presence of sub-lethal concentrations of rapamycin, in order to determine whether these proteins were potentially genetically linked to the TORC1 pathway. A majority of these proteins PI-103 showed altered sensitivity to growth in the presence of rapamycin. While this does not definitively show that these proteins associate with the TORC1, it suggests that these proteins are potentially linked to the TORC1 pathway. These data provided confidence in our purifications of the TORC1, and the identification of TORC1-interacting proteins. While these proteins are not a focus of this study, they drew our attention to the fourth group of identified proteins. This last subset of TORC1-associated proteins identified were all linked to various types of fungal filamentous growth.