This unique cultivar is also rich in theogallin, one of the bioactive factors that Reversine moa inhibited thrombin-induced MRLC phosphorylation in HUVECs and a novel inhibitor of IgE production as a promising anti-allergic target. These observations suggest that Nou-6 is an attractive green tea cultivar for ameliorating effects of stress and vascular function or for anti-allergic effects. However, this is a low-yielding cultivar, and it is difficult to grow. To overcome these problems, a new tea cultivar, Sunrouge , was generated by natural crossbreeding of Nou-6. This novel cultivar contains high levels of anthocyanins, grows vigorously, is high yielding and easy to grow, and shows high resistance to anthracnose and gray blight. Interestingly the bioactivities and metabolotypes of Nou-6 and SR are very similar. In the PCA score plot , Nou-6 and SR formed a group that was separate from the other 41 cultivars. In this plot , the confidence interval is defined by the confidence ellipse , and observations outside the confidence ellipse are considered outliers. If a sample shows a remarkably different metabolic profile from that of other samples it will fall outside the confidence ellipse. In the case of Nou-6 and SR, we repeatedly obtained similar results. This reproducibility suggested that Nou-6 and SR were unique cultivars with interesting compositional patterns, rather than outliers. We selected the SR cultivar for focused PCA analyses to elucidate detailed metabolic difference among tea cultivars. This was because of its interesting composition, but also because of its cultivation properties. The OPLS-DA results suggested that there were many differences in metabolites between SR and YB/BF. Almost all of the metabolite peaks focused by the two OPLS-DA overlapped with those removed by PVPP treatment. Considering the significance of the PVPP test to indicate bioactive groups in SR tea extract , it may be reasonable to use OPLS-DA for simple and high-precision screening for bioactive factors, without the need for additional experiments such as PVPP tests. These observations support the applicability of metabolic profiling with multivariate statistical analysis in nutraceutical MK-1775 research. Although some problems need to be solved, further metabolomic analyses and bioassays will increase our knowledge of the health promotion effects of the novel cultivar SR.