Here, we adopted a strategy of using HalT transporter as a query sequence to identify novel type IB lantibiotic clusters by microbial genome database mining. The strategy resulted in identification of 54 strains, from the top 72 hits that were encoding novel HalT homologs in the genome. Out of these 54 strains, we could identify 24 novel lantibiotic clusters that were encoding genes for precursor peptides, modification enzyme, quorum sensing, transport and immunity. Genome mining is being extensively and successfully done these days using bioinformatic tools, to identify novel genes and gene clusters. Recently, by taking advantage of the conserved nature of the genes encoded in the lantibiotic clusters. For example, LanC has been used as a query for the identification of type IA lantibiotic clusters, LanM for type IB clusters, C39 protease domain for double glycine motif containing precursor peptides and recently HylD and LanT was used for the identification of bacteriocins in cyanobacteria. In the present study,Basmisanil a similar approach was followed to identify novel lantibiotic biosynthetic clusters. HalT transporter of haloduracin biosynthetic cluster was taken as a query to screen the bacterial genomes on NCBI Entrez. HalT has a N-terminal C39 protease and a C-terminal ABCtransporter, for haloduracin precursor peptides. This strategy resulted in the identification of 59 novel LanT homologs in 54 bacterial genomes. The detailed characterization of all these LanT homologs and the nearby genome sequence, led to the identification of 24 novel lantibiotic clusters, described here. The bacteria encoding these clusters were the representatives of actinobacteria, firmicutes, cyanobacteria, proteobacteria and chloroflexi. Many examples of actinobacteria have been reported earlier for producing lantibiotics. This study led to the identification of five more actinobacteria encoding lantibiotic clusters namely S. hygroscopicus ATCC 53653, S. bingchenggensis BCW-1, S. viridochromogenes DSM 40736, S. roseosporus NRRL 11379 and Mycobacterium tusciae JS617. The three actinobacteria, ATCC 53653, BCW-1 and DSM 40736 were found encoding a putative two-component lantibiotic cluster, as suggested by the phylogenetic analysis of the precursor peptides and the presence of two LanMs. In ATCC 53653, the ORF encoding a second LanM was missed out in the genomic annotation while in BCW-1, it was hidden and fused with the LanT homolog. It was only the sequence identity among the precursor peptides, that gave us a clue for the possible presence of a second LanM. To draw a relation between the sequence identity among the precursor peptides and number of LanMs that are required for the processing, TLR7-agonist-1 examples of already known and putative lantibiotic clusters were taken and analysed. We could conclude therefrom that for upto 20% sequence identity, two LanMs are required for processing of the precursor peptides and above 37% identity, a single LanM is sufficient. From several studies done on lantibiotics, it has been felt that the search for smaller antimicrobial peptides is difficult because of their small size and low homology, and are therefore missed out during the conventional genomic annotation. However, the relatively larger transporter gene of the lantibiotic biosynthetic cluster i.e. LanT is rarely unannotated and can be identified by BLAST search.