This area corresponds both to the low AUC90 region on MRI and to the necrotic region on histology. Overall, the MRI and histological findings demonstrate that the LM2 is an orthotopic breast tumor model that requires little vascular support and yet can maintain rapid growth. Future studies will investigate spontaneous metastasis arising from a Povidone iodine primary LM2 breast tumor. The primary lesion will be removed 2 to 3 weeks after inoculation, depending on the Orbifloxacin growth rate in vivo, and the animal recovered. It is expected that successful xenografts will be established most of the time, given the high take rate achieved in this study. The time to appearance of metastatic lesions will need to be determined and may likely differ from the two months interval reported in mice. These studies will provide a platform for developing and applying new MRI methods for investigating and detecting breast cancer metastasis. In plants, the most common techniques to produce altered or loss of function mutations are based on insertional mutagenesisand RNA interference. However, because these methods are mainly based on the ability of a given plant to be transformed, their usefulness as general reverse genetics methods is limited to very few plant species and are unconceivable for species recalcitrant to plant transformation, such as cucumber. On the other hand, ethyl methanesulfonatemutagenesis is a simple method to saturate a genome with mutations. Targeting Induced Local Lesions in Genomescombines advantages of random chemical mutagenesis and high throughput mutation discovery methodsand generates allelic series of the targeted genes which makes it possible to dissect the function of the protein as well as to investigate the role of lethal genes. Furthermore, TILLinG produces a broad range of mutations including nonsense, missense and splicing mutations which can be used for protein domain annotation. This technique has been successfully applied to a large variety of organisms including plants and animalsand has become the method of choice for gene functional analysis in crop species. To assess the quality of the mutagenesis, we investigated the rate of appearance of depigmentation mutants at the cotyledon stage and developmental alterations at young seedling stage. Albino and chlorotic plants, the most frequently observed phenotype in mutagenized populations, occurred at the rate of 0.6% of the M2 families. This rate is similar to the rate reported for other well characterized mutant collections. The most commonly observed developmental phenotypes were related to cotyledon number and morphology, leaf shape and plant architecture. With the increasing skepticism against transgenic technology and growing transgenic crops, TILLinG, based on chemically induced mutagenesis, has become the method of choice for detailed gene functional characterization and mutation breeding for crop improvement as it yields a range of alleles with different phenotypic strengths. The identification of the optimal dose of a chemical mutagen that maximizes the mutation frequency with acceptable plant viability is a key factor for the establishment of a good TILLinG population. To set up the cucumber TILLinG platform, we first performed a preliminary “kill curve” analysis and observed a strong correlation between the EMS dose and the seed germination rate.